Red Blood Cell Antikörper

Produktnummer ABIN106454

Hersteller Produkt- Nr.: 203-4139

Der Kaninchen Polyklonal Anti-Red Blood Cell Antikörper wurde für AGG validiert. Er ist geeignet, Red Blood Cell in Proben von Huhn zu detektieren.

Kurzübersicht für Red Blood Cell Antikörper (ABIN106454)

Target: Red Blood Cell (RBC)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Red Blood Cell Antikörper ist unkonjugiert

Applikation: Agglutination (AGG)

Produktdetails anti-Red Blood Cell Antikörper

Hersteller Produkt- Nr.: 203-4139

Hersteller: Rockland

Verwendungszweck: Chicken Red Blood Cell RBC Antibody

Produktmerkmale: Synonyms: rabbit Anti-chicken RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti-chicken red blood cell, rabbit antibody to chicken red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrotyes, anti-erythrocytes antibody

Aufreinigung: This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Chicken washed pooled Red Blood Cells (RBC)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Tested for agglutination of cells on titer plates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Other: AGGLUTINATION TITER 1:32 - 1:64 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Red Blood Cell

Target: Red Blood Cell (RBC)

Andere Bezeichnung: RBC

Hintergrund: Background: Anti-CHICKEN Red Blood Cell Antibody may be used in hemagglutination assays. Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1 % to 0.7 % in steps of 0.2 %) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together. Anti-CHICKEN Red Blood Cell Antibody is used to sensitize erythrocytes and quantitate agglutination.